Procedure from collection to storage of adipose stem cells

Collection of adipose tissue and blood

Refrigerated transportation

Mycoplasma test

Hematology and liver function tests,Sterility test

Extraction of stem cells from tissue

Enzyme treatment and stem cell extraction

Culturing stem cells

Microscopic observation

Culturing cells

Changing of media and observing the culture

Storage and pre-tests

endotoxin, sterility, description, differentiation, endogenous viruses, identification, and purity (FACS)

Filling in vials

Storing in liquid nitrogen tanks